The CELENA S is a small, powerful digital imaging system that simplifies imaging and data analysis. Integrating advanced precision optics, a highly sensitive scientific-grade complementary metal oxide semiconductor (CMOS) camera, and a computer with user-friendly software, it allows researchers to capture vivid, publication-quality images with ease. Interchangeable objectives and filter cubes accommodate a wide range of imaging needs. Researchers can use the CELENA S for multiple applications, such as capturing and analyzing multicolor fluorescence images, live-cell imaging, and automated cell counting. The new onstage incubation system features an environmental chamber, temperature controller, and gas mixer. Researchers can control temperature, humidity, and gas content with precision. Live cells can be monitored with the time-lapse function or the growth monitor.