Q. How can I avoid bubbles in my sample being counted as cells?
A. Mix cells and loading buffer gently to avoid creating bubbles.
Q. What is the purpose of the QUANTOM™ Cell Loading Buffer I?
A. QUANTOM™ Cell Loading Buffer I is a viscous solution that immobilizes cells upon centrifugation.
Q. Why does it take so long to load the sample into the QUANTOM™ M50 Cell Counting Slide?
A. This is most likely due to the viscosity of the loading buffer. Leave the slide for 20-30 seconds after loading and try again. If it takes more than one minute, you may need to use a fresh batch of QUANTOM™ M50 Cell Counting Slides.
Q. What do I do if the slide is only partially full?
A. Load more of the sample into the slide (e.g. total 5.5-6.0 μL). If the slide does not fill completely with the increased volume, you may need to use a fresh batch of QUANTOM™ M50 Cell Counting Slides.
Q. What do I do if my cells aren’t all in the same focal plane? Some are in focus and some aren’t.
A. Increase centrifugation speed or time with the QUANTOM™ Centrifuge.
Q. What do I do if my cells are in the same focal plane but cell distribution is not even?
A. Lower centrifugation speed or time with the QUANTOM™ Centrifuge.
Q. Why did my cells clump after staining?
A. Increased dye incubation time can cause cell aggregation. Reduce incubation time and proceed quickly to counting.