Luna FL and Luna II : Trypan Blue cell counting tips

Use Trypan Blue to enhance the contrast for better accurate cell counting. A few critical tips may be useful for new users.

1. Mix 10 ul of cell samples with 10 ul Trypan Blue dye. Mix well by pipetting up and down a few times.

2. Load 10 ul of the mixture onto one of the counting chamber, either A or B.

3. Rest slide on a flat surface for 15 seconds to 30 seconds so the floating cell particles get a chance to settle down. This will help focusing.

4. Focus for a sharper and clear image: Adjusting focus by turning the focus knob or press up or down arrow on screen to get a better focused image. Use the magnifying glass so the small cells can be seen easily.

Clear well focused image will bring you reliable accurate counts.