Live Cell Sensitivity:Live cells with intact cell membranes exclude trypan blue and Erythrosin B. The dyes form halos around live cells and stain the cytoplasm of dead cells or cells with compromised membranes. With a higher live cell sensitivity, the instrument can detect smaller cells by registering smaller halos. For most cells, this option should be set to 1. For peripheral blood mononuclear cells (PBMCs)or smaller, set it to ³5.