Noise Reduction:

This refers to the decrease of the background for counting. Higher noise reduction will not detect faint signals and weakly stained objects. Lower noise reduction means increasing the sensitivity of the objects and detecting faint signals. Adjust the noise reduction to the appropriate level. It is also useful when cells are over-stained or under-stained with trypan blue stain.

Live Detection Sensitivity :

”Live Detection Sensitivity” was designed to detect the live cell having a various level of bright center. Increased “Live Detection Sensitivity” will detect more live cells. Decreased “Live Detection Sensitivity” will only detect very bright centered cells as live cells.  

Roundness:

This refers to the roundness of the objects on the image. Increasing the value requires more roundness of the objects to be included for the measurement. Meanwhile, decreasing the value includes objects that are less rounded for counting. Use this parameter when there are cell types that are not generally circular or are irregularly shaped.

Declustering Level:

There are 4 options for the level of declustering. This is useful for sticky cells or rod-shaped spore counting. High level declustering takes more analysis time