The Freedom Rocker BlotBot is an automated blot washer. Using a microprocessor for precise timing and dispensing, it automatically processes blots and gels in a consistent manner, every time. It frees you from babysitting your samples. Features Unattended dispensing and aspiration of liquids. Only requires an electrical outlet – no vacuum, pressurized air or gas, or additional pumps required. Can recover reagents, such as antibody solutions, separately from each gel or blot. Drip proof quick-release connections. Adjustable Parameters for each protocol step include: rocking speed (5 to 40 cycles per minute) reagent/solution rocking time (1 min to 90 hours) dispense volume (10 to 150 ml) up to 10 repetitions per step clearing rinse option Patent pending technology. Rocking continues after the final rinse and the wash buffer remains in the trays to prevent the blots or gels from desiccating Built in the USA for years of trouble free use. Uses existing protocols. Programming is easy and follows standard protocols steps. Programs can be up to 9 steps where each step is an immersion in a reagent (e.g. antibody) or a series of washes. In each step reagents or buffer will be dispensed and aspirated to all the trays simultaneously, for consistent processing of all the blots and gels. The minimum volume required to fully cover each mini blot is 3.5 mL. A clearing rinse can be activated to provide a quick rinse to get the final drop of reagent after each wash or reagent immersion. Quiet. The removable trays can be washed. Replacements can be ordered separately. A bottle of buffer is connected to the BlotBot with a hose and leak-proof quick-connector connections. 30-day money-back guarantee Two year warranty Specifications Number of Blots 1 – 4 mini blots or 1 – 2 midi blots Buffers 2 Buffer Volume Midi blot tray: 10 – 150 mL Mini blot tray: 10 – 75 mL per side Reagents Dispense: 2 Recover: 2 Reagent volume up to 50 mL each Minimum reagent per blot Mini blot: 3.5 mL (recover 3 mL) Midi blot: 7 mL (recover 6 mL) Protocol steps 1 – 9 Washes per step 1 – 10 Rocks per minute 5 – 40, independent for each step Free demonstration and evaluation is available.