The C-Chip is a disposable plastic hemocytometer used for manual cell counting. It consists of surface patterned with two enclosed chambers, each with a port for sample introduction.
The C-Chip grid pattern is the same as the Neubauer improved and Fuchs-Rosenthal patterns. It consists of 9 large squares, each measuring 1x1 mm, giving a total counting area of 9 square mm. The depth of the chamber is 0.1mm, giving a total volume of 0.9 cubic mm.
Disposable Hemocytometer C-CHIP pack of 50 Features:
Precise fixed depth counting chamber with superior accuracy and reproducibility.
No need to place cover glass on slide; possible to do precise quantification.
Reduces the risk of exposure to potentially infectious material.
Increases productivity by eliminating cleaning and work interruptions.
Very light and sturdy, compared with glass.
Ideal for teaching labs, animal hospitals and laboratories work with unkown infectious samples. Avoid the broken glasses of conventional hemocytometer and work in a safe environment.
Easy to count, simple to use. Eliminate the possibilities of getting in touch with unknown samples. Excellent for teaching labs, clinical and biology laboratories.
http://www.wisbiomed.com/dnld/Cchip_catalog.pdf">Download Brochure
How to Use
The DHC-N01 grid pattern is exactly same as the Neubauer Improved. It
consists of 9 large squares, each measuring 1?1 mm, and the depth of the
chamber is 0.1 mm. Each square has a total volume of 0.1 mm3 or 10-4 cm3.
The central square is divided into 25 small squares with triple lines and four
corner squares are divided into 16 small squares.
A. General Methods
1. Mix the samples well.
2. Load 10 ?l of sample into the sample injection area in Fig. 2, so that it fills
the chamber by capillary action.
3. Count the cells under the microscope.
Cells per ml = Average count per square x dilution factor x volume factor
B. Mammalian cell counting
1. Treat the cell samples with trypsin-EDTA.
2. Carefully remove the supernatant with a pipette tip without disturbing the
pellet.
3. Add an appropriate pp p volume of growth media or PBS to dilute to a final
concentration of 5?103 cells/ml to 5?106 cells per ml.
4. Thoroughly resuspend the cell pellet with a pipette.
5. Check visually if there are any cell clumps or agglomerates.
6. Load 10 ?l of sample into the sample injection area in Fig. 2.
7. Count the cells in 5 large squares.
Cells per ml =
cells in 5 large squares ×dilution factor×104 (volume factor)
5
× ×
C. Erythrocyte counting (1:200 dilution)
1 1. Dilute blood using accepted laboratory methods.
2. Load 10 ?l of diluted sample into the sample injection area in Fig. 2.
3. Count the erythrocytes in the 5 small squares (four small corner squares
and one small middle square) of the large center square.
RBCs per ml =
Cells in 5 small squares x 5 x 200 (dilution factor) x 104 (volume factor)
D. Leukocyte counting (1:20 dilution)
1. Dilute blood using accepted laboratory methods.
2. Load 10 ?l of diluted sample into the sample injection area in Fig. 2.
3. Count the leukocytes in the 4 large corner squares.
WBCs per ml =
cells in 4 corner squares ×20(dilution factor) ×104 (volume factor)
C-CHIPS disposable hemocytometer flyer
This product was added to our catalog on Saturday 06 February, 2010.
