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Disposable Hemocytometer C-CHIP pack of 50 for 100 tests

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The C-Chip is a disposable plastic hemocytometer used for manual cell counting. It consists of surface patterned with two enclosed chambers, each with a port for sample introduction.

The C-Chip grid pattern is the same as the Neubauer improved and Fuchs-Rosenthal patterns. It consists of 9 large squares, each measuring 1x1 mm, giving a total counting area of 9 square mm. The depth of the chamber is 0.1mm, giving a total volume of 0.9 cubic mm.

Disposable Hemocytometer C-CHIP pack of 50 Features:

  • Precise fixed depth counting chamber with superior accuracy and reproducibility.
  • No need to place cover glass on slide; possible to do precise quantification.
  • Reduces the risk of exposure to potentially infectious material.
  • Increases productivity by eliminating cleaning and work interruptions.
  • Very light and sturdy, compared with glass.

    Ideal for teaching labs, animal hospitals and laboratories work with unkown infectious samples. Avoid the broken glasses of conventional hemocytometer and work in a safe environment.

    Easy to count, simple to use. Eliminate the possibilities of getting in touch with unknown samples. Excellent for teaching labs, clinical and biology laboratories.

    http://www.wisbiomed.com/dnld/Cchip_catalog.pdf">Download Brochure

    How to Use The DHC-N01 grid pattern is exactly same as the Neubauer Improved. It consists of 9 large squares, each measuring 1?1 mm, and the depth of the chamber is 0.1 mm. Each square has a total volume of 0.1 mm3 or 10-4 cm3. The central square is divided into 25 small squares with triple lines and four corner squares are divided into 16 small squares.

    A. General Methods

  • 1. Mix the samples well.
  • 2. Load 10 ?l of sample into the sample injection area in Fig. 2, so that it fills the chamber by capillary action.
  • 3. Count the cells under the microscope. Cells per ml = Average count per square x dilution factor x volume factor

    B. Mammalian cell counting

  • 1. Treat the cell samples with trypsin-EDTA.
  • 2. Carefully remove the supernatant with a pipette tip without disturbing the pellet.
  • 3. Add an appropriate pp p volume of growth media or PBS to dilute to a final concentration of 5?103 cells/ml to 5?106 cells per ml.
  • 4. Thoroughly resuspend the cell pellet with a pipette.
  • 5. Check visually if there are any cell clumps or agglomerates.
  • 6. Load 10 ?l of sample into the sample injection area in Fig. 2.
  • 7. Count the cells in 5 large squares. Cells per ml = cells in 5 large squares ×dilution factor×104 (volume factor) 5 × ×

    C. Erythrocyte counting (1:200 dilution)

  • 1 1. Dilute blood using accepted laboratory methods.
  • 2. Load 10 ?l of diluted sample into the sample injection area in Fig. 2.
  • 3. Count the erythrocytes in the 5 small squares (four small corner squares and one small middle square) of the large center square. RBCs per ml = Cells in 5 small squares x 5 x 200 (dilution factor) x 104 (volume factor)

    D. Leukocyte counting (1:20 dilution)

  • 1. Dilute blood using accepted laboratory methods.
  • 2. Load 10 ?l of diluted sample into the sample injection area in Fig. 2.
  • 3. Count the leukocytes in the 4 large corner squares. WBCs per ml = cells in 4 corner squares ×20(dilution factor) ×104 (volume factor)

    C-CHIPS disposable hemocytometer flyer


    • Model: DB-CN1
    • 100 Units in Stock
    • Manufactured by: Digital Bio


    This product was added to our catalog on Saturday 06 February, 2010.

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